Select your language
We customize every project, but typically, this is how it goes:
Antigen Design: 1-2 weeks
Antigen Synthesis: multiple antigenic peptide (MAP) (4-6 weeks)
Day 0: Preimmune serum, initial injection of antigen and adjuvant
Day 18: Test sample and first booster. Subsequent boosters based upon ELISA titer.
Day 28: Begin collecting antiserum weekly.
Pricing examples:
Package 1: Antigen design and synthesis, immunization, ELISA, antiserum. $2000
Package 2: Package 1, plus antigen-specific purification. $5000
Our alpaca offerings are highly customized, depending upon whether you want PBMCs to construct a phage display VHH library, or polyclonal antisera.
Our experience suggests that proteins are the best antigens to use with alpacas. Once we have the protein, the typical schedule is similar to goats, but we find that the heavy chain antibody response takes longer to develop than the conventional antibody response.
Inquire for costs.
Coming Soon!
We can supply PBMCs for VHH libraries from naive alpacas upon request.
Availability varies, so please contact us to inquire.
Pricing:
PBMCs, shipped next day delivery in RNAlater. $5000
Inquire for other shipping formats!
We can also supply other alpaca serum or plasma products- if you are interested in something not listed here, then get in touch!
We use recombinant proteins or protein fragments for optimal heavy chain antibody response to conformational epitopes.
We design peptide antigens to minimize background and cross-reactivity. The MAP (multiple antigenic peptide) format enhances antigenicity to the peptide, without developing antibodies against the carrier protein.
Every antigen is a compromise between three factors: antigenicity (likelihood of generating an immune response), conservation (likelihood of detecting the protein in multiple species) and uniqueness (avoiding sequences present in non-target proteins to eliminate background). Your specific needs determine the emphasis placed on each factor.
Get StartedEvaluate protein alignments, antigenicity predictors, crystal structures, surface accessibility, and post-translational modifications to pick potential peptides.
We then evaluate the amino acid composition for factors that influence solubility, stability and ease of synthesis.
FInally, we localize the peptide candidates on 3-D structures, if possible, before picking the final peptide antigen for synthesis.
Finding an antibody is tough enough when you work with human, mouse or rat tissues. That challenge is magnified in other species. Our custom software will visually rank antigenicity, uniqueness and conservation in pigs, dogs and horses. Interested in another organism? We will happily design the antigen, using whatever information is available!
Get StartedAfter years at the bench, we noticed a common theme. Every researcher, at one time or another, struggles to find the perfect antibody. We started Talaria to make custom antibody production more accessible, whatever your research needs. We strive to be a partner at every step of the journey, from antigen design through purification. Collectively, we have decades of experience across multiple research fields and model organisms, with a passion for making your research easier.
To keep our alpacas in a climate more reminiscent of their ancestral home in the Andes, we opt for the colder climate in southern Canada. They are kept in a CCAC-approved environment, with handling and training protocols designed especially to minimize handling stress during injections and blood draws. Animals are brought indoors overnight and during inclement weather, and spend their days in a lovely pasture with a wind shelter and predator-resistant fencing.
Our goats are housed on dry lot pasture in the Southern US at an AAALAC-approved facility. They receive plenty of fresh air and sunshine! In addition, we focus closely on diet, continually innovating and updating to improve nutrition. B vitamins, selenium and vitamin E are particularly important, as is careful attention to hay quality. Goats are kept in a small flock to allow them the social interaction so integral to their natural behavior.
